I know someone else got the "Search failed" message before and I had some theories about what might have gone wrong, but I did not hear back so I am not sure if any of those theories were correct:
https://skyline.ms/announcements/home/support/thread.view?rowId=61146

If you would like, you could send us your files and we could see if we can figure out what is going wrong.
You could zip up all your files and upload them here:
https://skyline.ms/files.url

It would also be helpful if you could include some screenshots of the pages of the "Import Peptide Search" wizard so that we can see which settings you used when we try to reproduce your error.

I think in some of these cases the reason that the search failed was that the FASTA file was much too small. I am not sure what the minimum size of the FASTA file should be, but I imagine it should be a few hundred proteins at least.
-- Nick

Hi Nick,

Thanks for responding to my enquiry. I've uploaded the zip file (CalA.d.zip). This should contain the CalA.d (DDA data file), CalA.sky, CalA.fasta, and mzip.pptx (series of screenshots to replicate the error).

PS Please delete the CalA.zip folder as this does not contain the data file.

Thanks in advance!


Kind regards,
Elvis