It sounds like you wanted to attach some files to this support request but no files were actually attached.

This sometimes happens if the files you were trying to attach were more than 50MB.
You can upload larger files here:
https://skyline.ms/files.url
-- Nick

Thanks, Nick, for your prompt reply.
I've uploaded the raw files folder under the name " files for Skyline Support."

Best,
-Nitha

Can you also send us your Skyline document?
In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.
-- Nick

Hi Nick,

Here is the skyline document.


-Nitha

Thanks for attaching that Skyline document.

The m/z's of the precursors in your Skyline document are different from the Q1 values of the chromatograms in your raw file.
In the Skyline document, the m/z values are 768.4237 and 773.4368.
In your raw file, the Q1 values are 768.7 and 774

The setting which controls how close those numbers have to be for Skyline to consider them a match is "Method match tolerance m/z" at "Settings > Transition Settings > Instrument".
The maximum value that you can set that to is 0.6.
If you change that setting to 0.6 then you will see some chromatograms in your Skyline document, but a few transition chromatograms will still be missing because the Q3 value was different by more than 0.6.

Do you know why your mass spectrometer collected data for m/z values that were different than what Skyline thinks they should be?
-- Nick