Calibration Curve

support
Calibration Curve idoia beloqui-ezquer  2024-03-21 02:53
 

Dear Skyline support,

I'm using the Skyline interface for small molecules, and I came across one question about the calibration curves.

I do not have individual standards, instead, I have standards mixtures.
More than one of the mixtures contains the same molecule, and therefore I can build the calibration curve for the same molecule using several of the standard mixtures.

I would like to compare the calibration curves built using different standard mixtures for the same molecule.
Is it possible to build more than one calibration curve for one molecule?

Currently, I'm using the "Exclude From Calibration" in the "Document Gird" to select which standards I would like to use for the calibration of each molecule. However, I do not know how can I build several calibration curves for one molecule.

Sincerely,
Idoia

 
 
Nick Shulman responded:  2024-03-21 08:39
If you want to have multiple calibration curves in the Skyline document where each calibration curve is made from disjoint subsets of the replicates, then you should set the "Batch Name" on your different replicates.
The "Batch Name" is a column on Replicates that you can show in the Document Grid.

There is a PowerPoint showing how the Batch Calibration feature works here:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=BatchCalibration

If you need to learn more about using the Document Grid you should look at the Custom Reports tutorial:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports
-- Nick