Hi All,
I get a dotp and total ratio next to my precursor for the peptide of interest. I am aware that closer the value of dotp to 1, good is the matching.
However, I want to know how much shall be the TOTAL Ratio mentioned after that. Is there any particular value, i have to look for?
How much should be the spike-in heavy standards be injected as compared to endogeneous peptides?
For example, for my endogenous peptide transition, the intensity is 6e3 and for the corresponding heavy transition is 2e6 (screenshot attached).
Thank you.
VM