Skyline Targeted Proteomics Environment 
Register now to reserve your seat at the Skyline User Group Meeting at ASMS
    Baltimore, MD - Sunday, June 15th

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More Videos


New! Advanced Peak Picking Models

New! Custom and Live Reports (revised for v2.5)

New! MS1 Full-Scan Filtering (revised for v2.5)

New! Targeted MS/MS (PRM) (revised for v2.5)

New! Creating Chromatogram Libraries with Panorama

New! Sharing Skyline Documents with Panorama

New! Installable External Tools with Tutorials

New! SRM Course Tutorials

More Tutorials

Join Us

Summer ASMS 2-Day Courses in Baltimore, MD (June 14 & 15, 2014)

Summer Skyline User Group Meeting at ASMS 2014 - register now

Summer EU Proteomics Summer School Workshops in Brixen/Bressanone (August 3-9, 2014)

Fall Targeted Proteomics Course at PRBB Barcelona (Sep 28-Oct 3, 2014)


Spring US HUPO Short Course in Seattle, WA (April 6, 2014)

Spring Targeted Quantitative Proteomics Course at UW, Seattle (March 31-April 4, 2014)

Spring ABRF Workshop in Albequerque, NM (March 22, 2014)

Winter SRM Course at ETH Zurich (Feb 10-14, 2014) - review the tutorials

Panorama Webinar II, August 8, 2013 - watch the recording

Summer SRM Course at ETH Zurich (July 15-19, 2013) - review the tutorials

Skyline User Group Meeting at ASMS 2013 - review the presentations

Quantitative Proteomics Course at US HUPO (March 10, 2013) - review the workshop materials

Panorama Webinar, February 9, 2013 - watch the recording

Skyline User Group Meeting at ASMS 2012 - review the presentations

Register now!
Skyline User Group Meeting

Recently Published

Egertson, Nature Methods - Multiplexed MS/MS for improved data-independent acquisition

Abbatiello, Mollecular Cellular Proteomics - Design, Implementation, and Multi-Site Evaluation of a System Suitability Protocol for the Quantitative Assessment of Instrument Performance in LC-MRM-MS

Glukhova, Journal of Proteome Research - Rapid assessment of RNAi-mediated protein depletion by selected reaction monitoring (SRM) mass spectrometry

Nature Methods chose Targeted Proteomics as Method of the Year in 2012


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Get Involved

Make a tax-deductible contribution to the Skyline project this year through the University of Washington Foundation

See what the community is saying about Skyline - and add your statement!

Other ways you can help


Skyline adoption and use information

Create a new project for your lab or group on PanoramaWeb hosted by the University of Washington

Skyline source code is available under Apache 2.0 License and part of the ProteoWizard project (mzML and mzXML conversion)

Spectral Library Links